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Beyond Simple Colorimetry With MeasureNet Part 1


A typical colorimeter is usually a simple, single-beam optical system to measure “color” in the VISIBLE Spectrum of light and provide ABSORBANCE data at a single wavelength. These systems can cost $1,000 or more for stand-alone colorimeters that merely provide simple ABS data… unless you consider the MeasureNet Model MDBC-138 true Double-Beam, Multi-Functional “Colorimeter” based on a unique set of LEDs (Light-Emitting Diodes)... for under $500! The MeasureNet “Colorimeter” is actually FOUR (4) instruments in ONE (1) compact and rugged box… and works as a colorimeter (to make Beer’s Law Curves for VISIBLE Wavelengths), a UV fluorometer (to demonstrate fluorescence & quenching in certain organic molecules), a turbidometer (to measure the turbidity of particulate & colloidal suspensions) and a phosphorimeter (to analyze the phosphorescence [“glow”] of specific materials).

This unit can be used as a “simple” Colorimeter to demonstrate Beer’s Law using three (3) high-output VISIBLE Light LEDs at 472nm (BLUE), 525nm (GREEN) and 630nm (RED); which cover over 75% of the classic General Chemistry and Analytical Chemistry Laboratory experiments that teach spectrophotometric measurements. The wavelength coverage of these LEDs allows highly accurate relative measurement of almost all the ROYGBIV Colors. The disposable & unbreakable 10mm pathlength, near-UV plastic cuvettes included with the “Colorimeter” can be used from ~300nm (in the near-UV) to over 1000nm (in the near-IR) for analytical measurements… and come with sealing caps to preserve prepared solutions for future tests. Some of the popular Laboratory experiments defined in MeasureNet’s proprietary FUN-SCIence™ Laboratory Programs and its upcoming “Chem-Lab Library” that provide an excellent framework to create or supplement YOUR OWN Academic Lab curriculum program for Gen Chem, Analytical or Student Research are:

 Red / Blue / Green Colorimetric ABS Data for Beer’s Law plots:
• Test for Mineral IRON in your Daily “Eats”… check your Cereals, Breads and special Treats!
• Unsaturated FATS can be easily seen… just get a purple Color using some IODINE!
• Evaluation of Nutritional Food Proteins… React it to get a “BLUE” and see what can be seen!
• Consistency of M&M and Skittles Candy Colors: Is the "blue" true blue or “faux” to you!
• Changing “Colors” of some pH Dyes… [H+] makes them look *new* to our eyes!
• General Colorimetric Assay… for ANION (X, PO4, SO4, NO3, NO2, etc) saves the day!

Unlike most other popular Spectrophotometers… even those costing over $5,000… the MDBC-138 is a TRUE Double-Beam Optical System… and provides a reference cell to “blank” out the reagents used to create a very stable ABS reading for several Organic Reactions. Data from these Labs can be used to calculate rate constants, equilibrium factors and reaction conditions (for thermal, ionic and electrochemical variables). This is a MUST for making accurate kinetic experiment tests. A few of them from our “Library” include:

 Dual-Beam ABS Data for Kinetic Measurements:
• The Iodine Test for Starches is GREAT… just Hydrolyze with Amylaze to get a Kinetic Rate!
• Perform REDOX “Clock” Reactions in School… and show Students that Chemistry is Cool!
• pH-based Hydrolysis of p-nitrophenylacetate Ester… try it out in your Lab this Semester!



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